Anti-FMR1 (C-terminal) Antibody, clone 2B16 ZooMAb^® Rabbit Monoclonal

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 2B16 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Synaptic functional regulator FMR1. It targets an epitope within 18 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human Synaptic functional regulator FMR1.

应用

Affinity Binding Assay: A representative lot of this antibody bound FMR1 peptide with a KD of 7.0 x 10-8 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected FMR1 (C-terminal) in human testis tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected FMR1 (C-terminal) in HeLa, A431, HUVEC, and NIH 3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-FMR1 (C-terminal), clone 2B16 ZooMAb, Cat. No. ZRB1081, is a Rabbit monoclonal antibody that specifically targets FMR1 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

目标描述

Synaptic functional regulator FMR1 (UniProt: Q06787; also known as Fragile X mental retardation protein 1, FMRP, Protein FMR-1) is encoded by the FMR1 gene (Gene ID: 2332) in human. FMR-1 is a homodimeric multifunctional polyribosome-associated RNA-binding protein predominantly found in the brain. It can also form heterodimer with FXR1 in a methylation-dependent manner. It shuttles between nucleus and cytoplasm in a XPO1/CRM1-dependent manner and plays a central role in neuronal development and synaptic plasticity through the regulation of alternative mRNA splicing, mRNA stability, mRNA dendritic transport and postsynaptic local protein synthesis of a subset of mRNAs. FMR-1 plays a role in mRNA nuclear export and together with export factor NXF2, it is involved in the regulation of the NXF1 mRNA stability in neurons. It stabilizes the scaffolding postsynaptic density protein DLG4/PSD-95 and the myelin basic protein mRNAs in hippocampal neurons and glial cells, respectively and this stabilization is further increased in response to metabotropic glutamate receptor (mGluR) stimulation. In response to mGluR stimulation, FMR1-target mRNAs are rapidly derepressed, allowing for local translation at synapses. FMR-1 is shown to play a role in the modulation of the sodium-activated potassium channel KCNT1 gating activity and negatively regulate the voltage-dependent calcium channel current density in soma and presynaptic terminals of dorsal root ganglion (DRG) neurons. FMR-1 is phosphorylated on several serine residues and phosphorylation at serine 500 is required for phosphorylation of other nearby serine residues. Although phosphorylation has no effect on the binding of individual mRNA species, it may affect the association with polyribosome. Unphosphorylated FMR1 is reported to associate with actively translating polyribosome, whereas a fraction of phosphorylated FMR1 is associated with apparently stalled polyribosome. Mutations in FMR1 gene are linked to Fragile X syndrome that is characterized by moderate to severe mental retardation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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