位置:首页 > 品牌 > Sigma-Aldrich >

Anti-Rb Antibody, clone 2M15 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1014
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 2M15 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Retinoblastoma-associated protein (Rb). It targets an epitope within the C-terminal region.

免疫原

His-tagged recombinant fragment corresponding to 158 amino acids from the C-terminal region of human Retinoblastoma-associated protein (Rb).

应用

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Rb (Retinoblastoma associated protein) in HeLa, and NIH3T3 cell lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rb (Retinoblastoma associated protein) in HeLa, A431, HepG2 and NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Rb (Retinoblastoma associated protein) in human tonsil tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound recombinant Rb (Retinoblastoma associated protein) with a KD of 2.6 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Rb, clone 2M15 ZooMAb, Cat. No. ZRB1014, is a recombinant Rabbit monoclonal antibody that targets Retinoblastoma-associated protein and is tested Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting,

目标描述

Retinoblastoma-associated protein (UniProt: P06400; also known as p105-Rb, p110-RB1, pRb, Rb, pp110) is encoded by the RB1 gene (Gene ID: 5925) in human. Retinoblastoma (Rb) proteins act as a negative regulator of cell cycle and controls the passage of cells from G1 to S phase. It I a key regulator of entry into cell division that acts as a tumor suppressor. It acts as a transcription repressor of E2F1 target genes. In its underphosphorylated, active state it interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Its action is regulated by phosphorylation of several serine and threonine residues on the protein. Phosphorylated Rb protein sequesters transcription factors allowing the transition from G1 to S phase. It is phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at serine 567 in G1, thereby releasing E2F1 which is then able to activate cell growth. It is dephosphorylated in the late M phase. Phosphorylation at threonine 821 and 826 promotes interaction between the C-terminal domain and the Pocket domain (aa 373-771), and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Mutations in Rb1 gene are known to cause childhood retinoblastoma, a malignant tumor that arises from the nuclear layers of the retina. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in lyophilized from PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

0.03 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit (recombinant)
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆2M15, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 106.16 kDa
observed mol wt 120 kDa
species reactivityrat, human, mouse
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型IgG
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号