Anti-Calnexin Antibody, clone 10N19, ZooMAb^® Rabbit Monoclonal

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 10N19 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Calnexin. It targets an epitope within 20 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human Calnexin.

应用

Anti-Calnexin, clone 10N19 ZooMAb , Cat. No. ZRB1147, is a highly specific rabbit recombinant monoclonal antibody that targets Calnexin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin). and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Calnexin in Jurkat, NIH/3T3, and MCF7 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Calnexin in human tonsil and mouse testis tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Calnexin in HeLa, A431, HUVEC, NIH 3T3, Jurkat and MCF-7 cells.
Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.

目标描述

Calnexin (UniProt: P27824; also known as IP90, Major histocompatibility complex class I antigen-binding protein p88, p90) is encoded by the CANX gene (Gene ID: 821) in human. Calnexin is a member of the calreticulin family of proteins. It is a well-conserved, single-pass type I membrane protein that serves as calcium binding, endoplasmic reticulum (ER) resident chaperone protein. It associates with newly synthesized, incompletely folded monomeric glycoproteins and promotes their correct folding prior to secretion. It is also reported to target misfolded proteins for degradation. It is anchored to the ER membrane by a transmembrane domain. Calnexin associates with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes and may, therefore, function as a signaling complex regulating thymocyte maturation. Calnexin is synthesized with a signal peptide of 20 amino acids, which is subsequently cleaved off to generate the mature form that has a lumenal domain (aa 21-481), a short transmembrane domain (aa 482-502) and a cytoplasmic domain (aa 503-592). Three isoforms of calnexin have been described that are generated by alternative splicing. Calnexin is shown to be phosphorylated at serine 564 by MAPK3/ERK1, which increases its association with ribosomes. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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