Anti-PICH Antibody, clone 142-26-3

产品说明

一般描述

Plk1-interacting checkpoint helicase (PICH ) is an essential component of the spindle assembly checkpoint (Baumann, C., et al. (2007). Cell 128:101–114). This protein is an ATPase family SNF2 member, and serves as a binding partner and substrate for Plk1. When PICH is phosphorylated, it retrieves Plk1 which serves to mediate the localization of PICH. As a DNA helicase, PICH gathers at kinetochores and centromeres during prometaphase, acting as a necessary part of the spindle assembly checkpoint by recruiting MAD2 and mediating chromatin centromeric tension. Depleted PICH results in the cancellation of the spindle checkpoint causing large scale missegregation of the chromosome.

特异性

This antibody recognizes Plk1-interacting checkpoint helicase.

免疫原

Histidine-tagged recombinant protein corresponding to human Plk1-interacting checkpoint helicase.
Epitope: Unknown

应用

Research Sub Category
Cell Cycle, DNA Replication & Repair
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC.
Research Category
Epigenetics & Nuclear Function
Use Anti-PICCH Antibody, clone 142-26-3 (mouse monoclonal antibody) validated in WB, IP, ICC to detect PICCH also known as Tumor antigen BJ-HCC-15, PLK1-interacting checkpoint helicase, ATP-dependent helicase ERCC6-like.

质量

Evaluated by Western Blot in HeLa-S3 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Plk1-interacting checkpoint helicase on 10 µg of HeLa-S3 cell lysate.

目标描述

~160 kDa observed

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa-S3 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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