Anti-SAP130 Antibody, clone 1YO 1B10

产品说明

一般描述

SAP130, also known as Splicing factor 3B subunit 3, is part of the RNA splicing machinery. It is expressed by the Sf3b3 gene and encodes subunit 3 of the splicing factor 3b protein complex. As a component of this protein complex, it is associated with U2 snRNP and recruited to prespliceosomal complexes. Splicing factor 3b along with splicing factor 3a and a 12S RNA, form the U2 small nuclear ribonucleoprotein complex (U2 snRNP). In pre-mRNA’s, the splicing factor 3b/3a complex binds upstream of intron branch sites possibly anchoring the U2 snRNP to the pre-mRNA.
SAP130 is also a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes are believed to function in chromatin modification, transcription, splicing, and DNA repair.

特异性

This antibody recognizes SAP130.

免疫原

Ovalbumin-conjugated linear peptide corresponding to mouse SAP130.

应用

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected SAP130 in NIH/3T3 cells.
Use Anti-SAP130 Antibody, clone 1YO 1B10 (Mouse Monoclonal Antibody) validated in WB, ICC to detect SAP130 also known as 130 kDa Sin3-associated polypeptide, SF3b130.

质量

Evaluated by Western Blot in RAW264.7 cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected SAP130 on 10 µg of RAW264.7 cell lysate.

目标描述

~ 136 kDa

外形

Unpurified

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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