Anti-Akt2/PKBβ Antibody, clone AW114, rabbit monoclonal

产品说明

一般描述

Akt is the major known effector of the PI3 Kinase pathway. Generation of PIP3 results in the activation of PDK1, which phosphorylates Akt on Thr308, and another kinase complex, thought to be the mTORC2 complex (or possibly an intermediate kinase linking the two) that phosphorylates Akt on Ser473. These phosphorylations additively activate Akt Ser/Thr kinase activity, and the use of phosphorylation state-specific antibodies directed against either of these sites can imply Akt activation. Activation of Akt can be measured directly by immunoprecipitation followed by phosphorylation of a known substrate with radiolabeled ATP. Akt has been shown to phosphorylate over 70 substrates including TSC1&2 in the mTOR pathway, Bad on Ser136, GSK3 (which is inactivated by this phosphorylation), and the FoxO family of transcription factors (PRAS40, AS160).

特异性

Predicted to cross-react with mouse.
Recognizes Akt2/PKBβ, Mr ~60 kDa.

免疫原

KLH-conjugated, synthetic peptide (CRYDSLGSLELDQRTH) corresponding to amino acids 455-469 of human Akt2/PKBβ, with an N-terminal cysteine added for conjugation purposes.
Epitope: a.a. 455-469

应用

Anti-Akt2/PKBβ Antibody, clone AW114 detects level of Akt2/PKBβ & has been published & validated for use in WB & IP.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling

质量

Western Blot Analysis: A 1:500 dilution of this antibody detected Akt2/PKBβ in HepG2 lysate.

目标描述

~60 kDa

联系

Replaces: 04-406

外形

Cultured supernantant containing 0.05% sodium azide.
Format: Purified

储存及稳定性

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
L6 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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