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Anti-monomethyl-Histone H4 (Lys20) Antibody, clone NL314, rabbit monoclonal

品牌
Sigma-Aldrich
货号
04-735
规格纯度
culture supernatant, clone NL314, Upstate®
参考价格
6694.05 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications, which include acetylation, phosphorylation, methylation, and ubiquitin-ation, occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Trimethylation of histone H4 on lys20 (H4-K20me3) selectively marks constitutive pericentromeric hetero-chromatin and imprinting control regions, while mono- and dimethylation are broadly distributed but principally enriched in euchromatin regions. Although the biological function of H4K20me remains poorly understood, studies suggest that H4-K20me2 is involved in the guidance of DNA repair proteins to DNA strand breaks, whereas H4K20me1 is associated with chromatin condensation processes. PR-Set7 (also known as SET8) specifically catalyzes monomethylation of H4K20me1.

特异性

Recognizes monomethyl-histone H4 (Lys20), Mr ~10kDa.
The immunizing sequence is conserved from Tetrahymena to human, so broad species cross-reactivity is expected.

免疫原

KLH-conjugated, synthetic peptide containing the sequence …HR[mmK]V…, in which mmK corresponds to monomethyl-lysine at residue 20 of human histone H4.

应用

Use Anti-monomethyl-Histone H4 (Lys20) Antibody, clone NL314 (rabbit monoclonal antibody) validated in WB, Mplex to detect monomethyl-Histone H4 (Lys20) also known as H4K20me1, Histone H4 (mono methyl K20).
Immunoblot Analysis:
A 1:4000 dilution of a previous lot detected methylated histone H3 in acid extracted proteins from HeLa cells. The antibody did not detect unmethylated recombinant Histone H3.
Multiplexing:
Beadlyte Histone-Peptide Specificity Assay: 1:2,000-1:30,000 dilutions of a previous lot were incubated with histone H4 peptides containing various modifications conjugated to Luminex microspheres. Specificity to monomethyl-histone H4 (Lys20) was demonstrated (Figure C).
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function

质量

Routinely evaluated by immunoblot analysis.
Immunoblot Analysis:
A 1:1000-1:2000 dilution of this lot detected monomethyl histone H4 (Lys20) in HeLa acid extracts. (Figure A).

目标描述

10kDa

外形

Cultured supernantant in 0.05% sodium azide

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.

分析说明

Control
HeLa acid extracts

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式culture supernatant
antibody product typeprimary antibodies
克隆NL314, monoclonal
species reactivityhuman
manufacturer/tradenameUpstate®
technique(s)multiplexing: suitable
western blot: suitable
同位素/亚型IgG
NCBI登记号NM_175054
UniProt登记号P62805
运输dry ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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