Anti-Estrogen Receptor α Antibody, clone F3-A

产品说明

一般描述

Estrogen Receptor α (ERα) is a 65 kDa nuclear hormone receptor. This steroid hormone receptor is involved in the regulation of eukaryotic gene expression and affects cellular proliferation and differentiation in target tissues. ERα is a ligand-activated transcription factor and upon being bound by a ligand such as estrogen, a conformational change occurs that allows dimerization and binding to an estrogen response element. When bound to DNA, Estrogen Receptor α can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Two ERs are known, ERα, classically known as ER, and ERβ. The Estrogen Receptor is believed to play an important role in the growth and development of a subset of hormone dependent human breast cancers. ERβ is expressed in prostate, ovary, and other reproductive organs. Although its steroid specificity is very similar to that of the ERα, the molecular mechanisms regulating the transcriptional activity of ERβ may be distinct from those regulating ERα.

特异性

This antibody recognizes Estrogen Receptor α.

免疫原

Epitope: Unknown
Linear peptide corresponding to human Estrogen Receptor α.

应用

Research Sub Category
Transcription Factors
Anti-Estrogen Receptor α Antibody, clone F3-A is a mouse monoclonal antibody for detection of Estrogen Receptor α also known as Estradiol receptor & Nuclear receptor subfamily 3 group A member 1 has been validated in WB, ICC.
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Estrogen Receptor α in A431 cells.

质量

Evaluated by Western Blot in mouse ovary tissue lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Estrogen Receptor α on 10 µg of mouse ovary tissue lysate.

目标描述

~ 67 kDa

外形

Unpurified
Unpurified mouse monoclonal IgG1κ preservative-free ascites.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
Mouse ovary tissue lysate

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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