产品说明
一般描述
An antibody that recognizes the C-terminus of histone H3 and is useful for identifying total H3 independant of post-translational modifications.
特异性
Recognizes Histone H3.
Broad species cross-reactivity expected due to sequence homology
免疫原
KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3
应用
Anti-Histone H3 Antibody, CT, pan, clone A3S is a Rabbit Monoclonal Antibody for detection of Histone H3 also known as Histone H3 & has been validated in ChIP & WB.
Western Blot Analysis: 1:500-1:2,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.
Chromatin Immunoprecipitation:
2 μL of of a previous lot of this antibody immunoprecipitated chromatin associated with Histone H3 from a wild type yeast lysate.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
质量
Routinely evaluated by immunoblot analysis.
目标描述
17 kDa
联系
Replaces: 05-928
外形
Cultured supernantant in 0.05% sodium azide
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | culture supernatant |
| antibody product type | primary antibodies |
| 克隆 | A3S, monoclonal |
| species reactivity | human, Saccharomyces cerevisiae, mouse, rat, chicken |
| manufacturer/tradename | Upstate® |
| technique(s) | ChIP: suitable western blot: suitable |
| 同位素/亚型 | IgG |
| NCBI登记号 | NM_003493 |
| UniProt登记号 | Q16695 |
| 运输 | dry ice |
| Gene Information | human ... H3F3B(3021) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
