产品说明
一般描述
The Brachyury protein is a T-box transcription factor that is one of the earliest indicators of mesoderm formation during embryonic development. As the brachyury protein family is essential for the formation of the posterior body in all vertebrates, Brachyury has been shown to be expressed during gastrula and neurula stages, as well as in the developing notochord. Consequently, when mutations occur within the brachyury gene, severe vertebral malformations have been found to occur, as a direct result. In addition, current findings also indicate that the dysregulation of brachyury may also be linked to the formation of chordomas, which are malignant tumors that develop along the spine.
特异性
Reactivity with other species has not been tested.
This monoclonal antibody recognizes the brachyury protein.
免疫原
Synthetic human brachyury peptide, a.a. 224-435.
Epitope: C-terminus
应用
Research Sub Category
Mesenchymal Stem Cells
This Anti-Brachyury Antibody, clone 3E4.2 is validated for use in WB for the detection of Brachyury.
Research Category
Stem Cell Research
质量
Routinely evaluated by Western blot on NT2 cell lysates.
Western Blotting:
Recommended working dilutions are 1:1000.
目标描述
47 kDa
外形
Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.
Format: Purified
Protein G Purified
储存及稳定性
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 3E4.2, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Upstate® |
| technique(s) | western blot: suitable |
| input | sample type mesenchymal stem cell(s) |
| 同位素/亚型 | IgG1κ |
| NCBI登记号 | NP_003172 |
| UniProt登记号 | O15178 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
