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Monoclonal Anti-Ryanodine Receptor antibody produced in mouse

品牌
Sigma-Aldrich
货号
R129
规格纯度
clone 34C, ascites fluid
参考价格
10871.5 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

The ryanodine receptor (RyR) is the channel responsible for the release of Ca2+ from the sarcoplasmic reticulum (SR) in muscle cells and it also plays a role in Ca2+ regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR-1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR-2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).
The antibody reacts strongly with RyR-1 (expressed predominantly in skeletal muscle and areas of the brain; also known as the α isoform) and RyR-2 (expressed predominantly in the heart muscle, but also found in stomach, endothelial cells and diffuse areas of the brain; also known as the β isoform). In immunohistochemistry, the antibody stains are consistent with the endoplasmic reticulum localization of the receptor in rat hippocampus.

免疫原

partially purified chicken pectoral muscle ryanodine receptors (RyR)

应用

Mouse monoclonal clone 34C anti-Ryanodine receptor is used to tag ryanodine receptors for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of ryanodine receptor in studies of calcium flux from the sarcoplasmic reticulum of muscle cells.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

外形

Purified IgG1 antibody containing 0.05% sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD00282461
NACRESNA.41

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式ascites fluid
antibody product typeprimary antibodies
克隆34C, monoclonal
分子量antigen 565 kDa (non-mammalian vertebrates, doublet at 565 kDa representing the α and β isoforms)
species reactivityrabbit, fish, canine, sheep, frog, bovine, primate, mink, chicken, rat, human, mouse
technique(s)direct immunofluorescence: suitable
immunohistochemistry (frozen sections): 1:1,000
immunoprecipitation (IP): suitable
western blot: 1:5,000
同位素/亚型IgG1
UniProt登记号P21817
运输dry ice
储存温度−20℃
Gene Informationhuman ... RYR1(6261), RYR2(6262)
mouse ... Ryr1(20190), Ryr2(20191)
rat ... Ryr1(114207), Ryr2(84025)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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