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Monoclonal Anti-Rab9 antibody produced in mouse

品牌
Sigma-Aldrich
货号
R5404
规格纯度
~2 mg/mL, clone Rab9-29, purified immunoglobulin, buffered aqueous solution
参考价格
5045.46 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Monoclonal Anti-Rab9 (mouse IgG1 isotype) is derived from the hybridoma Rab9-29 produced by the fusion of mouse cells (NS1 cells) and splenocytes from BALB/c mice. Ras-related protein Rab-9A (Rab9) is a member of the Rab family of small guanosine triphosphatases (GTPases). The gene Rab9 is mapped to human chromosome Xp22.2. Rab9 GTPase is localized predominantly to late endosomes.

特异性

Monoclonal Anti-Rab9 recognizes human, canine, rat, mouse and hamster Rab9 (approx. 23 kDa)

免疫原

synthetic peptide corresponding to amino acids 187-200 of human Rab9, conjugated to KLH.

应用

Monoclonal Anti-Rab9 antibody produced in mouse has been used in:

  • western blotting
  • enzyme-linked immunosorbent assay(ELISA)
  • immunoprecipitation
  • immunocytochemistry

生化/生理作用

Ras-related protein Rab-9A (Rab9) guanosine triphosphatase (GTPase) is required for the transport of mannose 6-phosphate receptors (MRPs) from endosomes to the trans-Golgi network (TGN). Rab GTPases are central regulators of membrane trafficking between the different subcellular compartments of the eukaryotic cell. Rab9 GTPase in its GTP bound conformation binds specifically to an effector protein named p40 that stimulates the transport of mannose 6-phosphate receptors (MPRs) from endosomes to the TGN. Rab9 also interacts with the vesicle cargo selection protein tail-interacting protein of 47 kDa (TIP47). This interaction is required for Rab9 stability on late endosomes and may be crucial for vesicular transport in the virus life cycle.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

储存及稳定性

For continuous use, store at 2-8 ℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD03097193
NACRESNA.41

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆Rab9-29, monoclonal
形式buffered aqueous solution
分子量 ~23 kDa
species reactivityhuman, rat, canine, mouse, hamster
浓度~2 mg/mL
technique(s)immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 2 μg/mL using total cell extract of HeLa cells.
同位素/亚型IgG1
UniProt登记号P51151
运输dry ice
储存温度−20℃
Gene Informationhuman ... RAB9A(9367)
mouse ... Rab9(56382)
rat ... Rab9a(84589)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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