产品说明
一般描述
Trans-Golgi network integral membrane protein 2 (TGOLN2), also known as trans-Golgi network 46 (TGN46), is encoded by the gene mapped to human chromosome 2p11.2. It is a heterodimeric type I integral membrane protein. TGOLN2 has a highly conserved N terminus, which consists of a signal peptide. The C terminus possesses a lumenal domain, a membrane spanning region and cytoplasmic tail.
免疫原
synthetic peptide corresponding to the C-terminal region of human TGN46, conjugated to KLH. The corresponding sequence is identical in monkey and differs by 3 amino acids in rat and mouse TGN46.
生化/生理作用
Trans-Golgi network integral membrane protein 2 (TGOLN2) cycles between the trans-Golgi network (TGN) and the cell surface via an early endosomal compartment. This movement is mediated by a tyrosine-based tetra peptide signal (SDYQRL) in the cytoplasmic domain. It plays an important role in the formation of exocytic vesicles at the TGN by functioning as a receptor for complexes of a cytoplasmic protein known as p62 and one GTP-binding protein. The cytoplasmic domain of TGOLN2 binds to the complex and is essential for budding process. It mediates the coupling of the segregation of secretory proteins to the budding of exocytic vesicles. The cytosolic domain of TGOLN2 interacts with AP2 clathrin adaptor complexes and also with the coiled coil region of a protein called neurabin.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD09265378 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | TGN46-8, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | 80-100 kDa |
| species reactivity | human |
| 浓度 | ~1.0 mg/mL |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using HeLa cells western blot: 2-4 μg/mL using whole extracts of HEK-293T cells over-expressing human TGN46 |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | O43493 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... TGOLN2(10618) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
