产品说明
一般描述
Monoclonal Anti- β -Catenin (mouse IgG1 isotype) is derived from the 6F9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. CTNNB1 (catenin β 1) gene is mapped to human chromosome 3p21. The protein encoded is also called as cadherin-associated protein.The encoded protein β-catenin is localized to the nucleus. Monoclonal antibody reacting specifically with β -catenin is an essential tool in defining the interactions and distributions of β -catenin and its relationships with other catenins and cadherins in various cells and tissues.
特异性
The antibody does not cross-react with plakoglobin (γ-catenin).
免疫原
recombinant chicken β-catenin.
应用
Monoclonal Anti-β-Catenin antibody produced in mouse has been used in:
- immunohistochemistry
- immunofluorescence
- immunoblotting
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
生化/生理作用
In Wnt/β-catenin signal pathway, β-Catenin participates as a signal transduction molecule. The pathway is associated with development, tissue homeostasis and oncogenic transcription. Upregulation of the gene is associated with glioma cell proliferation. The encoded protein has dual function that regulates the coordination of cell-cell adhesion by linking cytoskeleton and cellular junctions and gene transcription. Wnt/β-catenin signal pathway is known to induce progression of colon cancer progression. It is useful for differential diagnosis of Osteoblastoma (OB) and osteosarcoma (OS). β-catenin signaling pathway may contribute to cholangiocarcinoma (CCA) cell proliferation. Monoclonal antibody reacting specifically with β -catenin is an essential tool in defining the interactions and distributions of β -catenin and its relationships with other catenins and cadherins in various cells and tissues.
外形
Supplied as ascites fluid with 15 mM sodium azide as a preservative.
储存及稳定性
For extended storage freeze in working aliquots. For continuous use, store at 2-8 ℃. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD00282178 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | ascites fluid |
| antibody product type | primary antibodies |
| 克隆 | 6F9, monoclonal |
| 分子量 | antigen 94 kDa |
| 包含 | 15 mM sodium azide |
| species reactivity | human, canine, chicken, bovine |
| technique(s) | indirect immunofluorescence: 1:1,000 using cultured MDBK cells microarray: suitable western blot: 1:1,000 using cultured MDBK cells extract |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P35222 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... CTNNB1(1499) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
