产品说明
一般描述
The MAGUK (Membrane Associated Guanylate kinase) family of proteins localize to regions of cell-cell contact, such as tight junctions in epithelial cells and synaptic junctions in neurons. In epithelia, membrane associated guanylate kinase, WW And PDZ domain containing 1 (MAGI-1) is localized at tight junctions and is expressed in three isoforms (MAGI-1a, MAGI-1b, and MAGI-1c). MAGI-1 was first identified in mouse as a protein interacting with K-RasB (Ras-like protein rasB).
特异性
Recognizes rat MAGI-1 by immunoblotting (approx. 170, 130, and 120 kDa) and does not cross react with MAGI-2 or MAGI-3.
免疫原
synthetic peptide corresponding to amino acids 282-296 of MAGI-1 with a carboxy terminal added cysteine residue conjugated to maleimide-activated KLH. The sequence is conserved in human and mouse.
应用
Anti-MAGI-1 antibody produced in rabbit has been used in
- immunoblotting
- western blotting
- immunostaining
生化/生理作用
Membrane associated guanylate kinase, WW And PDZ domain containing 1 (MAGI-1) interacts with β-catenin. Neuroepithelial cell-transforming gene 1(nNET1) and actin binding proteins act as binding partners of MAGI-1. Interestingly, MAGI-1c is present in nucleus, suggesting that MAGI-1 may participate in the transmission of regulatory signals from the cell surface to the nucleus.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD04040441 |
| NACRES | NA.46 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| species reactivity | rat |
| technique(s) | microarray: suitable western blot: 1:500 using rat brain extracts |
| UniProt登记号 | Q96QZ7 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... MAGI1(9223) mouse ... Magi1(14924) rat ... Cnksr3(308113) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
