产品说明
一般描述
Monoclonal Anti-Phosphotyrosine (mouse IgG1 isotype) is derived from the PT-66 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with phosphotyrosine-BSA conjugate.
经 ELISA 和竞争性 ELISA 确定，该抗体与磷酸化的酪氨酸特异性反应，该磷酸化的酪氨酸既可以是游离氨基酸形式，也可与 BSA 或 KLH 等载体结合的形式。与非磷酸化的酪氨酸、磷酸苏氨酸、磷酸丝氨酸、AMP 或 ATP 没有观察到交叉反应。
特异性
Monoclonal Anti-Phosphotyrosine is specific for phosphorylated tyrosine both as the free amino acid or when conjugated to carriers such as BSA or KLH. It does not react with non-phosphorylated tyrosine or other phosphorylated amino acids, including serine
免疫原
与 BSA 结合的磷酸酪氨酸
应用
Monoclonal Anti-Phosphotyrosine has been used in
- immunoblotting,
- immunofluorescence,
- immunohistochemistry
- immunocytochemistry
- flow cytometry
- immunoprecipitation
- enzyme linked immunosorbent assay (ELISA)
- radio immunoassay (RIA)
- immunoaffinity isolation
生化/生理作用
Protein phosphorylation is a basic signaling mechanism that modifies protein function in eukaryotic cells. Serine, threonine, and tyrosine are the major phosphorylated amino acids in proteins. Tyrosine phosphorylation is a rare post-translational event in normal tissues, accounting for only 0.03% of phosphorylated amino acids. However, this phosphorylation increases several folds by various activation signals and the process is mediated by protein tyrosine kinases. Protein-tyrosine kinases (PTKs) are enzymes that catalyze the transfer of γ-phosphate of ATP to tyrosine residues of protein substrates. The PTKs are responsible for many biological processes like cell cycle, proliferation, oncogenesis, and development.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.44 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | PT-66, monoclonal |
| 形式 | buffered aqueous solution |
| 包装 | antibody small pack of 25 μL |
| 浓度 | ~2 mg/mL |
| technique(s) | flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable indirect ELISA: 0.5-1.0 μg/mL using phosphotyrosine conjugated to BSA radioimmunoassay: suitable western blot: 0.25-0.5 μg/mL using total cell extract of human platelets |
| 同位素/亚型 | IgG1 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
