产品说明
细胞系来源
Human Caucasian colon adenocarcinoma
细胞系描述
Established from a grade III adenocarcinoma of the bowel of a 51 year old Caucasian female. The cells produce CEA and are tumorigenic in nude mice. Electron microscopy reveals prominent golgi apparatus.
应用
Tumourigenicity studies
培养基
L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; No CO2; 37℃. Cells do not reach confluency but will grow in small islands.
其他说明
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产品性质
生物来源 | human colon |
生长模式 | Adherent |
核型 | 2n = 46, triploid |
形态学 | Epithelial |
产品 | Carcinoembryonic antigen (CEA) |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |
储存温度 | −196℃ |