产品介绍：
产品说明
细胞系来源
Human Caucasian foetal lung, SV40 transformed
细胞系描述
An SV40 transformed derivative of WI 38. The cells are characterised by loss of contact inhibition, unlimited proliferation and the presence of SV40 antigens.
应用
Transformation and virus studies: SV40
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
其他说明
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产品性质
| 生物来源 | human lung (fetal) |
| 生长模式 | Adherent |
| 核型 | 2n = 46, hyperdiploid, modal no. 73-78 |
| 形态学 | Epithelial |
| 产品 | Not specified |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
| 储存温度 | −196℃ |
