产品说明
细胞系来源
Human lymphoblast
细胞系描述
Derived from a patient with myelomonocytic leukaemia and subsequently exposed to Epstein Barr Virus (EBV). This line is EBV positive and therefore presumably derived from a normal B lymphocyte. This cell line is also positive for surface Ig, Fc receptor, complement receptor, surface Ia and EBNA. Responds to T cell replacement factor by increasing the number of IgG secreting cells. The cells should be handled under laboratory containment level 2.
应用
T cell replacement factor (TRF) assays, B cell differentiation studies
DNA图谱分析
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,9
vWA: 16,17
培养基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37℃.
其他说明
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产品性质
生物来源 | human blood |
生长模式 | Suspension |
核型 | Not specified |
形态学 | Lymphoblast |
产品 | Surface Immunoglobulin ( Ig), MHC Class II |
受体 | Fc, complement |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |
储存温度 | −196℃ |