LB173

产品说明

细胞系来源

Human lymphoblastoid, normal,EBV-transformed

细胞系描述

Derived from mononuclear cells from peripheral blood of a 49 year old normal male. Cells were used as controls for cellular radiosensitivity studies and analysis of cell survival after UV-irradiation. The line LB173 has been established by subsequent transformation with EBV. Fibroblasts from the same individual have been designated 144BR.

应用

Mutation studies

培养基

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

传代培养常规

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO₂, 37℃. Cells form floating aggregates.

其他说明

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产品性质