产品介绍：
产品说明
细胞系来源
Human Caucasian colon adenocarcinoma
细胞系描述
Derived from a 58 year old female Caucasian with Dukes type B adenocarcinoma of the colon. The cells secrete CEA and mucin and are tumorigenic in nude mice. LS174T (Sigma Catalogue number. 87060401) was derived from the same patient. The cells have not been exposed to trypsin.
应用
Tumourigenicity studies
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 by scraping; 5% CO2; 37℃.
其他说明
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产品性质
| 生物来源 | human colon |
| 生长模式 | Adherent |
| 核型 | Modal no. 45 (2n=46) |
| 形态学 | Epithelial-like |
| 产品 | Carcinoembryonic antigen (CEA), Mucin |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 相关疾病 | cancer |
| 运输 | dry ice |
| 储存温度 | −196℃ |
