产品说明
细胞系来源
Human placenta, SV40 transformed
细胞系描述
Established by SV40 ts30 transformation of human placental cells. The cells express the transformed phenotype at the permissive temperature (33C) and the non-transformed phenotype at the non-permissive temperature (40C). The line has a limited life expectancy of 15 to 18 passages before entering the crisis stage.
GMO1
培养基
α-MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-6x10,000 cells/cm2 using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO2; 36℃. Change medium 2-3 times weekly.
其他说明
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产品性质
| 生物来源 | human placenta |
| 生长模式 | Adherent |
| 核型 | Hypodiploid, modal chromosome number of 42 occuring in 34% of cells. Most cells contained 41-45 chromosomes. Cells with 44 chromosomes also occurred at a high frequency (26%). Cells having a higher ploidly occurred at 20.7%, which is very high. |
| 形态学 | Epithelial |
| 产品 | At 40C cells synthesize human chorionic gonadotrophin (hCG) and alkaline phosphatase. |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 相关疾病 | cancer |
| 运输 | dry ice |
| 储存温度 | −196℃ |
