SV-T2

产品说明

细胞系来源

Mouse BALB/3T3 clone A31

细胞系描述

Derived by SV40 transformation of the line BALB/3T3 clone A31. The cells exhibit a high saturation density, lack contact inhibition and form tumours in appropriate hosts.

应用

Transformation, tumourigenicity and virus studies: SV40

培养基

DMEM + 2mM Glutamine + 10% New Born Calf Serum (NBCS) or (5% NBCS + 5% Foetal Bovine Serum (FBS)).

传代培养常规

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1x1,000 to 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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产品性质