RT112/84

产品说明

细胞系来源

Human bladder carcinoma epithelial

细胞系描述

A slow growing tumour which does not form colonies in soft agar. Tumorigenic in nude mice, RT1112/84 cells possess a low plating efficiency requiring 400 cells/dish to form any colonies.

应用

RT112/84 cancer cell line has been used to study the cytotoxic effect of lipoic acid and calcium hydroxycitrate concentrations on RT112/84 cells.
Tumourigenicity studies

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 13,14
D16S539: 11,13
D5S818: 10,13
D7S820: 11,12
THO1: 7
TPOX: 8,11
vWA: 14,17

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37℃. Subculture weekly - cells are difficult to trypsinise, and should be kept sub-confluent.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

产品性质