产品说明
应用
据报道，来自欧洲帽贝的酶比来自盖罩大蜗牛、牛肝脏和大肠杆菌的酶能更有效地水解阿片肽-葡糖苷酸
比较不同酶性能的新技术文章
了解更多关于 Sigma R&D 生成的最新应用数据 使用不同来源的酶优化不同药物类别的水解，并使用色谱纯化酶降低酯酶活性的影响，导致 6-MAM 转化为吗啡
Poster presentation comparing the hydrolysis efficiency of novel beta-glucuronidase enzymes from recombinant limpet and recombinant E. coli sources, click at the following poster link: Hydrolysis Efficiency Evaluation of Novel Recombinant Limpet and E. coli Beta-Glucuronidase Enzymes
More on the suitability of this product for hydrolysis of different drug-glucuronide substrates is available at this page.
特点和优势
Recombinantly expressed and purified to eliminate MAM esterase activity (6-Monoacetylmorphine→morphine). Representative analysis at high enzyme titer (50 un/μL β-glucuronidase) was less than 1% conversion of 6-MAM to morphine after 4 hours at 60 ℃.
单位定义
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 ℃ at pH 3.8 (30 min assay).
外形
Supplied as a solution in 100mM potassium acetate pH 5.2. It is free of detergents, carbohydrates or any other components that may interfere with sample preparation and analysis.
基本信息
| 酶学委员会(EC)编号 | 3.2.1.31 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD00130629 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in proprietary host |
| 形式 | aqueous solution |
| 浓度 | 100000-200000 units/mL |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
