产品说明
细胞系来源
Canine Cocker Spaniel kidney
细胞系描述
Derived from the kidney of a normal adult Cocker Spaniel in 1958. Useful in viral studies, supports the growth of vesicular exanthema virus of swine, vesicular stomatitis, vaccina, coxsackie B-5, adenoviruses, reoviruses and infectious canine hepatitis. Also known as MDCK (NBL-2).
应用
Virus studies: SVEV, VSV, Vaccinia, reoviruses and inf. canine hepatitis virus
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37℃. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
基本信息
eCl@ss | 32190102 |
产品性质
生物来源 | dog kidney |
生长模式 | Adherent |
核型 | 2n = 78 |
形态学 | Epithelial-like |
产品 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
运输 | dry ice |
储存温度 | −196℃ |