产品说明
一般描述
2′-Deoxycytidine 5′-triphosphate (dCTP) is synthesized either by de novo pathway or from the multiple phosphorylation steps of cytidine. It is deaminated to deoxyuridine monophosphate (dUMP) which is convertible to deoxythymidine triphosphate (dTTP).
应用
2′-脱氧胞苷 5′-三磷酸二钠盐是用于 DNA 聚合酶驱动反应如聚合酶链反应、DNA 测序等分子生物学方法的三磷酸核苷之一。
2′-Deoxycytidine 5′-triphosphate disodium salt has been used:
- in polymerase chain reaction mix for the amplification of Alternaria (AM) toxin DNA
- to spike oligonucleotide sample for capillary electrophoresis (CE) analysis
- as a component in adenosine 5′-monophosphate (AMP) luciferase assay
包装
10 mg in glass bottle
25, 100 mg in poly bottle
1 g in poly bottle
生化/生理作用
2′-Deoxycytidine 5′-triphosphate (dCTP) is used to synthesize DNA by DNA polymerases or reverse transcriptases. dCTP is an allosteric regulator of deoxycytidylate (dCMP) deaminase. It is used in studies of nucleotide pools, nucleotide selectivity and enzyme specificity studies, such as the preferred incorporation of dCTP by Rev1, a γ-family DNA polymerase.
基本信息
| 经验(实验)分子式 | C9H14N3Na2O13P3 |
| 分子量 | 511.12 |
| MDL编号 | MFCD00084683 |
| PubChem化学物质编号 | 24893889 |
| NACRES | NA.51 |
产品性质
| 质量水平 | 200 |
| 测定 | ≥95% |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| SMILES string | [Na+].[Na+].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)O2 |
| InChI | 1S/C9H16N3O13P3.2Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;/q;2*+1/p-2/t5-,6+,8+;;/m0../s1 |
| InChI key | ABWVCNMFYVEBIB-CDNBRZBRSA-L |
安全信息
| 储存分类代码 | 4.1B - Flammable solid hazardous materials |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
