产品说明
细胞系来源
Mouse helper T cell
细胞系描述
Derived in 1979 from BALB/c spleen cells activated by sheep erythrocytes in the presence of IL-2, the A5E clone was selected to provide a homogenous population that is highly sensitive to IL-2. This cell line expresses Thy-1 and Lyt markers in addition to high affinity IL-2 receptors. Substantial proliferation is seen in the presence of IL-4 (GM-CSF).
应用
IL-2 assays
培养基
RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 100-200 IU/ml IL-2 + 10% Foetal Bovine Serum (FBS).
传代培养常规
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37℃. When starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
产品性质
| 生物来源 | mouse blood |
| 生长模式 | Suspension |
| 核型 | Not specified |
| 形态学 | Lymphoblast |
| 产品 | Not specified |
| 受体 | Interleukin 2 (IL-2), Interleukin 4 (IL-4) |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
| 储存温度 | −196℃ |
