产品说明
细胞系来源
Human colon carcinoma
细胞系描述
Cells possess Ca antigen as defined by the Ca1 antibody. The Ca antigen has been shown to be absent in non-malignant human cell strains and is considered to be a marker linked with malignancy. Island cell growth will degenerate if left more than 9 days between passages.
应用
Tumourigenicity studies
DNA图谱分析
STR-PCR Data: Amelogenin: X
CSF1PO: 7,12
D13S317: 13
D16S539: 10
D5S818: 12
D7S820: 11,12
THO1: 6,9
TPOX: 8
vWA: 18
培养基
DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37℃. Cells grow relatively slowly, in islands.
其他说明
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产品性质
生物来源 | human colon |
生长模式 | Adherent |
核型 | Not specified |
形态学 | Epithelial |
产品 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |
储存温度 | −196℃ |