产品介绍：
产品说明
细胞系来源
Rat hepatoma
细胞系描述
Derived from a rat ascites tumour, which in turn was derived from a solid hepatoma induced in male Buffalo rats by feeding them 0.04% N,N′-2,7fluorenyle-bis-2,2,2-trifluoroacetamide for 12.4 months.
应用
Tumourigenicity and enzymatic studies
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37℃.
其他说明
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产品性质
| 生物来源 | rat liver |
| 生长模式 | Adherent |
| 核型 | 2n = 66, hypertriploid |
| 形态学 | Epithelial |
| 产品 | Not specified |
| 受体 | Dexamethasone phosphate; hydrocortisone |
| technique(s) | cell culture | mammalian: suitable |
| 相关疾病 | cancer |
| 运输 | dry ice |
| 储存温度 | −196℃ |
