产品说明
一般描述
Thrombin is a sodium-activated type II enzyme. It contains two anion binding exosites, ABE-I and ABE-II. The predominant form of thrombin in vivo is the zymogen prothrombin (factor II), which is produced in the liver. Bovine a-thrombin consists of a light chain (A chain) and a heavy chain (B chain). These two chains are joined by one disulfide bond. The B chain of a-thrombin includes a carbohydrate portion.
应用
Thrombin from bovine plasma has been used to study its effect on the perinatal rat subventricular zone cells and oligodendrocyte precursor cell proliferation, differentiation, and migration in culture. It has also been used in fibrin degradation assay to measure nattokinase activity.
凝血酶用于重组融合蛋白的位点特异性切割，所述重组融合蛋白包含用于去除亲和标签的可接近凝血酶识别位点。凝血酶已被用于一项评估全球止血和护理点测试的研究。
生化/生理作用
Thrombin is a proteolytic enzyme critical in the blood clotting process and activates clotting factors V, VIII, XI, and XII. Thrombin promotes platelet aggregation. Therefore, thrombin is the final coagulation protease in hemostasis, promoting both procoagulant and anticoagulant effects. It is used to treat bleeding from capillaries and small venules.
丝氨酸蛋白酶，选择性断开纤维蛋白原中的精氨酸-甘氨酸键，形成纤维蛋白以及纤维蛋白肽 A 和 B。
单位定义
活性以NIH单位表示，通过与NIH凝血酶参考标准品直接对比而获得。
外形
由生理盐水柠檬酸钠缓冲液，pH 6.5，制得冻干粉
分析说明
活性是通过与一种K批次的NIH凝血酶参考标准品进行直接比较而得到并以NIH单位进行表达的。
基于 Biggs 改性法的 NIH 分析过程使用 0.2mL 稀释血浆(由血浆与等量生理盐水混合制得)作为底物，以及 0.1mL 凝血酶样品(在含 1% 白蛋白缓冲液中保持稳定)。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。
其他说明
查看更多关于 凝血酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer。
基本信息
| 酶学委员会(EC)编号 | 3.4.21.5 ( BRENDA | IUBMB ) |
| EC 号 | 232-648-7 |
| MDL编号 | MFCD00166053 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 300 |
| 形式 | lyophilized powder |
| specific activity | 600-2,000 NIH units/mg protein (biuret) |
| 分子量 | heavy chain ~33 kDa light chain ~5 kDa |
| UniProt登记号 | P00735 |
| application(s) | diagnostic assay manufacturing |
| 储存温度 | −20℃ |
| Gene Information | cow ... F2(280685) |
安全信息
| 象形图 |   |
| 警示用语： | Danger |
| 危险声明 | H315 - H319 - H334 - H335 |
| 预防措施声明 | P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| 危险分类 | Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3 |
| 靶器官 | Respiratory system |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
