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Lectin from Ulex europaeus (gorse, furze)

来自 荆豆的凝集素

品牌
Sigma-Aldrich
货号
L8146
规格纯度
peroxidase conjugate, lyophilized powder
参考价格
3510.56 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

包装

0.5, 1 mg in serum bottle
Package size based on protein content.

应用

General Western Blot Protocol:

  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

生化/生理作用

荆豆凝集素具有抗H血型特异性。凝集素有两种类型: UEA I对 L-岩藻糖具有亲和力,UEA II对N,N′-二乙酰基壳二糖具有亲和力。最初认为UEA I摩尔量为170,000道尔顿。 后来的报道表明,UEA I可以在中性和碱性pH下形成聚集体,正确的摩尔量是68,000道尔顿。

联系

由亲和纯化凝集素制备偶联物。

单位定义

One unit will form 1.0 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 ℃.

外形

Contains sodium citrate buffer salts

制备说明

Prepared from peroxidase, Type VI (P8375), using the two-step glutaraldehyde method of Avrameas and Ternyck.
Repurified by affinity chromatography after conjugation.

分析说明

凝集活性以μg/ml表示,由磷酸盐缓冲液(pH 6.8)连续稀释1 mg/ml溶液而测得。该活性是指25℃孵育1小时后使2%人O型血红细胞悬浮液凝集的最低浓度。

基本信息

NACRESNA.32

产品性质

生物来源Ulex europaeus
质量水平200
偶联物peroxidase conjugate
测定70.00-90.00% (Lowry)
形式lyophilized powder
效能<40 μg/mL agglutination activity
peroxidase activity≥15 units/mg protein
组成Protein, ~80% Lowry
technique(s)western blot: suitable
储存温度2-8℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, type N95 (US)

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