产品介绍：
产品说明
包装
0.5, 1 mg in serum bottle
Package size based on protein content.
应用
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
生化/生理作用
荆豆凝集素具有抗H血型特异性。凝集素有两种类型： UEA I对
联系
由亲和纯化凝集素制备偶联物。
单位定义
One unit will form 1.0 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 ℃.
外形
Contains sodium citrate buffer salts
制备说明
Prepared from peroxidase, Type VI (P8375), using the two-step glutaraldehyde method of Avrameas and Ternyck.
Repurified by affinity chromatography after conjugation.
分析说明
凝集活性以μg/ml表示，由磷酸盐缓冲液(pH 6.8)连续稀释1 mg/ml溶液而测得。该活性是指25℃孵育1小时后使2％人O型血红细胞悬浮液凝集的最低浓度。
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | Ulex europaeus |
| 质量水平 | 200 |
| 偶联物 | peroxidase conjugate |
| 测定 | 70.00-90.00% (Lowry) |
| 形式 | lyophilized powder |
| 效能 | <40 μg/mL agglutination activity |
| peroxidase activity | ≥15 units/mg protein |
| 组成 | Protein, ~80% Lowry |
| technique(s) | western blot: suitable |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
