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LCFC-LCAT Acyltransferase Activity Assay

LCFC-LCAT 脂肪酰转移酶活性检测试剂盒

品牌
Sigma-Aldrich
货号
MAK306
规格纯度
Supplied by Roar Biomedical, Inc., sufficient for 100 fluorometric tests
参考价格
9335.36 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol forming cholesteryl ester. This activity occurs on the surface of high density lipoprotein (HDL) and the cholesteryl esters formed by LCAT are incorporated into the core of HDL.

特点和优势

  • Non-enzymatic determination of cholesteryl ester formation.
  • Not affected by LCAT inhibitors or other chemical compounds.
  • Dilution or non-dilution type assay.
  • Assay components stable for up to one year.
  • Compatible with high-throughput handling systems.

适用性

Suitable for high-throughput screening, mechanism of action studies and structureactivity relationship (SAR) work of LCAT in plasma and serum

原理

The LCFC-LCAT Acyltransferase Activity Assay is a fluorometric assay useful for measuring the acyltransferase activity of LCAT in serum or plasma. The method detects changes in LCAT free cholesterol (LCFC) levels in the sample without the use of cholesterol oxidase, peroxidase, or the generation of hydrogen peroxide. Detection is not affected by iodoacetate or other LCAT inhibitors. LCAT activity results in a fluorometric (λEx = 320 nm/λEm = 405 nm) product proportional to the amount of free cholesterol present.

基本信息

NACRESNA.84

产品性质

用途 sufficient for 100 fluorometric tests
detection methodfluorometric
储存温度2-8℃

安全信息

储存分类代码12 - Non Combustible Liquids
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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