产品说明
一般描述
Monoclonal Anti-Rabbit Immunoglobulins (mouse IgG1 isotype) is derived from the RG-16 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG. Immunoglobulins (Igs) belongs to the immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L) chains held together by disulfide linkages. Each light comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is abundant immunoglobulin in human serum. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4.
Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.
特异性
Binds only rabbit Igs. Does not react with reduced rabbit Igs.
应用
The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.
Mouse monoclonal anti-rabbit immunoglobulins have been used for western blot assays at a dilution of 1:1000 using bovine trabecular meshwork cell lysates. The antibody can also be used for immunohistochemistry assays (1:100, using human tissue).
Monoclonal Anti-Rabbit Immunoglobulins-Peroxidase antibody produced in mouse has been used in:
- western blotting
- immunoblotting
- immunoprecipitation
- enzyme linked immunosorbent assay (ELISA)
- immunohistochemistry
外形
Lyophilized from 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT
制备说明
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.46 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | peroxidase conjugate |
| 抗体形式 | purified immunoglobulin |
| antibody product type | secondary antibodies |
| 克隆 | RG-16, monoclonal |
| 形式 | lyophilized powder |
| species reactivity | rabbit |
| should not react with | goat, bovine, human, canine, chicken, horse, feline, sheep, rat, turkey, guinea pig, pig |
| 包装 | vial of 0.5 mL |
| technique(s) | direct ELISA: 1:30,000 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissue western blot (chemiluminescent): 1:160,000 |
| 同位素/亚型 | IgG1 |
| 储存温度 | 2-8℃ |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H317 |
| 预防措施声明 | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| 危险分类 | Skin Sens. 1 |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
