产品说明
一般描述
EZviewä Red Glutathione Affinity Gel is a highly visible, red colored glutathione agarose affinity gel. The affinity resin is composed of glutathione covalently attached through the sulfur to epoxy-activated 4% cross-linked agarose beads, resulting in a 12-atom spacer.
应用
The product is used for affinity capture, molecular pull-down, or IP of proteins containing glutathione binding sequences, such as glutathione S-transferase, glutathione peroxidase and glyoxalase I.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview™ resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
包装
1 mL in poly bottle
特点和优势
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
外形
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
法律信息
EZview is a trademark of sigma-aldrich Co. LLC
基本信息
MDL编号 | MFCD05665380 |
NACRES | NA.56 |
产品性质
质量水平 | 200 |
analyte chemical class(es) | proteins (GST) |
technique(s) | immunoprecipitation (IP): suitable protein purification: suitable |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 3 |