产品说明
一般描述
Epo is mainly produced by liver cells in fetal stages, by kidneys under hypoxic conditions; liver and brain also secrete Epo in less amounts. Anti-Erythropoietin antibody specifically reacts with human Erythropoietin. The EPO gene is mapped to human chromosome 7q22.1.
免疫原
CHO 细胞中表达的重组人促红细胞生成素。
应用
Anti- Erythropoietin antibody may be used for immunoblotting at a working concentration of 1 μg/mL. For immunohistochemistry, a working concentration of 5-15 μg/mL may be used. The antibody is also suitable for neutralization reactions. Erythropoietin antibody has been used for ELISA in the extracts of leaves of tobacco plant in which recombinant human erythropoietin was expressed. The antibody has also been used for immunoaffinity chromatography to separate the leaf extracts of tobacco expressing recombinant human erythropoietin.
生化/生理作用
Erythropoietin (Epo) is a pleotropic cytokine that promotes erythropoiesis or proliferation and differentiation of red blood cell precursors. The red blood cell production is stimulated when Epo binds and activates the Epo receptor (EpoR) dimer. EpoR directly regulates the signalling by Jak2, PI3K, NF-κB, STAT5A and STAT5B. Epo also inhibits apoptosis of erythroid progenitors by downregulating Fas (tumor necrosis factor receptor superfamily member 6) and FasL (Fas ligand). The expression of Epo is under the control of the transcription factor hypoxia inducible factor (HIF). Erythropoietin exhibits disease-modifying effects in infectious diseases and chronic inflammatory disorders Anti-Erythropoietin antibody specifically reacts with human Erythropoietin.
外形
在含碳水化合物的磷酸盐缓冲盐水中用0.2μm滤过溶液冻干。
免责声明
除非我们的目录或随附于产品的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
基本信息
| MDL编号 | MFCD00676455 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | lyophilized powder |
| species reactivity | human |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5-15 μg/mL neutralization: suitable western blot: 1 μg/mL |
| UniProt登记号 | P01588 |
| 储存温度 | −20℃ |
| Gene Information | human ... EPO(2056) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
