产品说明
应用
Chaperonin 60 from Escherichia coli has been used:
- in mass spectroscopy
- in cryo-electron microscopy imaging as control for testing particle distribution
- as standard in infrared spectrum measurements
包装
1 mg in serum bottle
Package size based on protein content.
生化/生理作用
Chaperonin 60 (GroEL) and chaperonin 10 (GroES) belong to the ubiquitous family of heat-shock molecular chaperones found in prokaryotes and in eukaryotic organelles. The chaperonins assist the folding of nascent, organelle-imported or stress-destabilized polypeptides. In vitro, purified GroEL together with purified GroES in the presence of Mg-ATP facilitate refolding and reactivation of denatured proteins, e.g., the photosynthetic enzyme rubisco and the mitochondrial enzyme rhodanese.
The folding activity of a 1:1 molar mixture of GroEL and GroES was tested using urea-denatured rhodanese. At least 2-fold reactivation of rhodanese over the spontaneous reactivation was obtained.
外形
Lyophilized powder containing Tris buffer salts, potassium chloride, magnesium chloride, dithiothreitol, and trehalose as stabilizer.
基本信息
| MDL编号 | MFCD00163256 |
| NACRES | NA.32 |
产品性质
| 生物来源 | Escherichia coli |
| 质量水平 | 200 |
| 重组 | expressed in E. coli overproducing strain |
| 测定 | >95% (SDS-PAGE) |
| 形式 | lyophilized powder |
| technique(s) | electron microscopy: suitable mass spectrometry (MS): suitable |
| UniProt登记号 | P10809 |
| 储存温度 | 2-8℃ |
| Gene Information | human ... HSPD1(3329) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
