T47D/S2

产品说明

一般描述

Oestrogen receptor T47DS2 cells express ER alpha and progesterone receptor.

细胞系描述

T47D/S2 cells have been established by reduction of the serum concentration to 2% fetal calf serum, in order to enable growth inhibition with tamoxifen. The T47D/S2 Cell line is the control cell line for the tamoxifen resistant T47D/TR-1 and T47D/TR-2 lines.

培养基

RPMI 1640 w/o Phenol Red (R7509) + 2% FBS (F2442) + 2.5 mM Ala-Gln (G8541) + 8μg/ml Insulin (I9278)

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10, 000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37℃. Recommend media change every 2-3 days

其他说明

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

引用

If use of this culture results in a scientific publication, it should be cited in the publication as: T47D/S2 (ECACC 16022512)

免责声明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

产品性质