产品说明
一般描述
TrkA belongs to the Trk proto-oncogene family and is expressed mainly in central and peripheral nervous systems. When TrkA binds to NGF, it autophosphorylates tyrosine residues like-Tyr490, Tyr674, Tyr675, Tyr751, and Tyr785 and subsequently activates many downstream effector proteins. Defects in TrkA lead to congenital insensitivity to pain with anhidrosis. Anti-phospho-TrkA (pTyr490) antibody can be used in immunocytochemistry (1:100, using cultured cells). It is also useful in identifying endogenous levels of phosphorylated Tyr490 in TrkA, TrkB and TrkC. Rabbit anti- phospho-TrkA (pTyr490) antibody is reactive in mouse, human and rat.
免疫原
synthetic phosphopeptide Tyr490 corresponding to residues surrounding Tyr490 of human TrkA, conjugated to KLH. This sequence is highly conserved in TrkB and TrkC.
应用
Anti-phospho-TrkA (pTyr490) antibody can be used in immunoprecipitation, immunoblotting and immunohistochemistry. It may also be used in western blotting when diluted to ratio of 1:1000 for NGF-treated PC12 cells.
外形
Solution in 10 mM sodium HEPES, pH 7.5, and 150 mM sodium chloride containing 100 μg/ml bovine serum albumin and 50% glycerol.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD01092507 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous glycerol solution |
| 分子量 | antigen 140 kDa |
| species reactivity | human, mouse, rat |
| technique(s) | immunocytochemistry: 1:100 using cultured cells immunohistochemistry (frozen sections): 1:10 immunoprecipitation (IP): 1:250 western blot: 1:1,000 using NGF-treated PC12 cells |
| UniProt登记号 | P04629 |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... NTRK1(4914) mouse ... Ntrk1(18211) rat ... Ntrk1(59109) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
