位置:首页 > 品牌 > Sigma-Aldrich >

Aminopeptidase I from Streptomyces griseus

品牌
Sigma-Aldrich
CAS
9031-94-1
货号
A9934
规格纯度
lyophilized powder, ≥200 units/mg protein
参考价格
3330.91 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Aminopeptidase I from Streptomyces griseus is a thermostable enzyme with Glu131 and Tyr246 as key active site residues.

应用

Aminopeptidase I from Streptomyces griseus has been used:

  • to test the biochar exposure effect on the enzyme activity
  • in circular dichroism (CD) spectroscopy studies
  • as a positive control in p-nitroanilide degradation assay

包装

0.1 mg in serum bottle
Package size based on protein content.

生化/生理作用

Aminopeptidase I may also be used as a reagent in the assay of endoprotease activities with a synthetic substrate in a two-stage assay. In the first stage, the endoprotease cleaves a peptide, such as Z-Y-X-Leu-p-nitroanilide, with the X, Y, and Z residues being chosen according to the specificity of the endoprotease.
Aminopeptidase I from S. griseus has a fairly broad specificity, being able to remove the N-terminal residue of most proteins, except where the penultimate residue is an imino acid. It contains two Zn2+ binding sites. Aminopeptidase I from S. griseus is inhibited by 1,10-phenanthroline and is activated six-fold by Ca2+, which also stabilizes it against heat inactivation. This monomeric zinc metalloprotein has an isoelectric point (pI) of 5.4.

单位定义

One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 8.0, 25 ℃ and 3.0 mM substrate concentration.

外形

Contains calcium acetate

制备说明

Reconstitute in 20 mM tricine, pH 8.0, with 0.05% bovine serum albumin. Dilute the enzyme with the reconstitution buffer to 0.15-0.3 U/mL for a working concentration. Solutions should be prepared fresh prior to use.

其他说明

Endopeptidase contaminant: Not more than: 0.01 U/mg protein (as μmole tyrosine equivalent per min released from casein.)

基本信息

酶学委员会(EC)编号3.4.11.22 ( BRENDA | IUBMB )
EC 号232-874-6
MDL编号MFCD00166325
NACRESNA.54

产品性质

形式lyophilized powder
质量水平200
specific activity≥200 units/mg protein
分子量 21 kDa by gel filtration
33 kDa by SDS-PAGE
组成Protein, 40-60% Lowry
储存温度−20℃

安全信息

象形图GHS07GHS08
警示用语:Danger
危险声明H315 - H319 - H334 - H335
预防措施声明P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
危险分类Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官Respiratory system
储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, type N95 (US)

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号