产品说明
一般描述
Alkaline phosphatase, a metalloenzyme is a member of ectonucleotidases family. It is present in bile ducts, bone, intestine, placenta and tumors.
应用
Alkaline Phosphatase Blue Membrane Substrate Solution has been used:
- to analyse direct alkaline phosphatase (AP) activity
- to perform alkaline phosphatase assays with colorimetric system
- to analyse direct alkaline phosphatase (AP) activity of the induced pluripotent stem cells (iPSC) line LND554SV.3
Prior to reaction with alkaline phosphatase, the BCIP® reagent is colorless to faint blue and the Nitro BT reagent is yellow. The two-component mixture develops a bluish-purple product with alkaline phosphatase in membrane-type assays. This substrate is not recommended for ELISA assays.
外形
A 2 component kit sufficient for preparing 400 mL of working substrate.
Supplied as a two-component buffered alkaline phosphatase substrate containing bromochloroindolyl phosphate (BCIP®) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)
法律信息
BCIP is a registered trademark of sigma-aldrich Co. LLC
基本信息
eCl@ss | 42010105 |
NACRES | NA.83 |
产品性质
质量水平 | 200 |
形式 | solution |
用途 | sufficient for 400 mL working substrate |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
闪点(F) | Not applicable |
闪点(C) | Not applicable |