产品说明
一般描述
Mitogen-Activated Protein Kinase Kinase 4 (MEK 4 or MKK4) is a 42 kDa member of a tyrosine/threonine protein kinase family that activates the c-Jun NH2-terminal kinases (JNK), which is part of the inflammation/stress signaling pathway. The mutation of the MEK 4 gene in some carcinomas indicates that it may also have a role as a tumor suppressor. Phosphorylation of MEK4 by MEKK1 on serine 257 and threonine 261 in the catalytic domain activates the protein and enables MEK 4 to phosphorylate JNK
This antibody specifically recognizes human MEK 4 double phosphorylated at serine 257 and threonine 261. Mouse and frog MEK 4 (100% homology) may cross react.
免疫原
synthetic MEK4-derived phosphopeptide that is phosphorylated at serine 257 and threonine 261.
应用
Anti-phospho-MEK4 (Tyr397) antibody may be used for detection by immunoblotting at a working concentration of 0.1-1.0 μg/mL.
外形
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.
基本信息
| MDL编号 | MFCD06798497 |
| NACRES | NA.44 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| species reactivity | frog, mouse, human |
| technique(s) | western blot: 0.1-1.0 μg/mL using extracts prepared from GST-tagged fusion protein expressing MEK 4. |
| UniProt登记号 | P45985 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... MAP2K4(6416) mouse ... Map2k4(26398) |
