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PhosphoDetect Anti-GSK3β (pSer9) Mouse mAb (2D3)

品牌
Sigma-Aldrich
货号
361527
规格纯度
liquid, clone 2D3, Calbiochem®
参考价格
8539.21 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
This PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3) is validated for use in ELISA, Immunoblotting for the detection of GSK3β (pSer⁹).
Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.

免疫原

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β
Human

包装

1 set in Plastic ampoule

应用

ELISA (0.05 µg/ml)

Immunoblotting (0.5 µg/ml)

警告

Toxicity: Standard Handling (A)

外形

100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.

重悬

Reconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.

分析说明

Positive Control
Pervanadate-treated HepG2 (included)

其他说明

For immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆2D3, monoclonal
形式liquid
包含≤0.1% sodium azide as preservative (antibody only)
species reactivitymouse, human
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
同位素/亚型IgG1
运输wet ice
储存温度−20℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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