产品说明
一般描述
A cell-permeable, potent, and relatively specific ATP-competitive inhibitor of CK1 (IC50 = 200 nM from S. pombe; 0.3 µM for CK1δ) and ALK5 (IC50 = 500 nM). A weak inhibitor of p38α MAP kinase (IC50 = 12 µM) and several other kinases tested, including PKB, SGK, and GSK-3β. Suppresses the site-specific phosphorylation (Ser322 and Ser325) and nuclear exclusion of FOXO1A in H4IIE hepatoma cells. Shown to be ~10-fold more potent than IC261 (Cat. No. 400090; IC50 = 2.5 µM for CK1).
包装
1 mg in Plastic ampoule
Packaged under inert gas
Please refer to vial label for lot-specific concentration.
生化/生理作用
Target IC50: 0.2 µM, 0.3 µM against CK1, CK1d, respectively
Primary Target
CK1, CK1d
Cell permeable: yes
Reversible: no
Product competes with ATP.
警告
Toxicity: Irritant (B)
外形
A 10 mM (1 mg/251 µl) solution of Casein Kinase I Inhibitor, D4476 (Cat. No. 218696) in DMSO
重悬
Following initial use, aliquot and refrigerate (4°C).
其他说明
Rena, G., et al. 2004. EMBO Reports5, 60.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
经验(实验)分子式 | C23H18N4O3 |
分子量 | 398.41 |
产品性质
质量水平 | 100 |
测定 | ≥95% (LC/MS) |
形式 | liquid |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze desiccated (hygroscopic) protect from light |
运输 | wet ice |
储存温度 | −20℃ |