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TrueGel3D Hydrogel Kits

TrueGel3D 水凝胶试剂盒

品牌
Sigma-Aldrich
货号
TRUE3
规格纯度
FAST-DEXTRAN, allow cell recovery, CD cell-degradable crosslinker
参考价格
3258.92 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

The gel is formed by stable thioether bonds between maleimide groups of dextran (FAST-DEXTRAN) and thiol groups on CD cell-degradable crosslinker. Malemide-functionalized dextran is specifically used to decrease the time for gel formation. The dextran polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1UM) prior to the crosslinking step. The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).
TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.
TrueGel3D® provides a high degree of control in a 3D hydrogel environment, providing greater consistency. It enables to fine-tune the microenvironments around the tissues or cells under study.

应用

TRUE3-1KT is used for cell migration studies of amoeboid cells including neutrophils, dendritic cells and lymphocytes. It has been used to study the effect of mechanic stimulus over tumor development, tumor progression and cell differentiation.

特点和优势

  • Fast gelling (takes few seconds to minutes after addition of crosslinker)
  • Contains MMP-cleavable crosslinker
  • Allow cell recovery and post-culture analyses
  • Flexibility in modulating gel stiffness
  • Cells do not attach to hydrogel (if not customized with TrueGel3D RGD peptide)

组分

TRUE3-1KT contains lyophilized FAST-DEXTRAN (polymer) and CD cell-degradable crosslinker with reconstitution buffer, TrueGel3D thioglycerol and water.

注意

Avoid prolonged exposure of CD cell-degradable crosslinker to air.

法律信息

TRUEGEL3D is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

运输dry ice
储存温度−70℃

安全信息

象形图GHS05
警示用语:Warning
危险声明H290
预防措施声明P234 - P390
危险分类Met. Corr. 1
储存分类代码8A - Combustible, corrosive hazardous materials

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