TM4小鼠睾丸支持细胞
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目录号 Serial | GNM41 | 标识符 Identifier | CSTR:19375.09.3101MOUGNM41 | 描述 Description | 据报道,TM4细胞株对FSH有响应,引起cAMP产出增加,但对黄体化激素(LH)没有响应。 与原代培养睾丸支持细胞相比,对FSH的响应显著降低。 据报道,组成型纤溶酶原产量低,但可被FSH诱导,更扩展点,被维甲酸诱导。 检测表明肢骨发育畸形病毒(鼠痘)阴性。 | 动物种别 Organism | 小鼠 | 性别 Gender | 雄 | 组织来源 Tissue and Cell Type | 器官:睾丸 | 形态 Morphology | 上皮细胞 | 培养基和添加剂 Complete Growth Medium and Culture Conditions | D-MEM/F-12培养基(GIBCO,货号11320-033),92.5%;马血清,5%;优质胎牛血清,2.5%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。 | 供应限制 Permits and Restrictions | A。仅供研究之用。 | REFERENCE | 1158: Mather JP. Establishment and characterization of two distinct mouse testicular epithelial cell lines. Biol. Reprod. 23: 243-252, 1980. PubMed: 67747811159: Mather JP, et al. Culture of testicular cells in hormone-supplemented serum-free medium. Ann. N.Y. Acad. Sci. 383: 44-68, 1982. PubMed: 70465611184: Carson DD, et al. Synthesis and secretion of a novel binding protein for retinol by a cell line derived from Sertoli cells. J. Biol. Chem. 259: 3117-3123, 1984. PubMed: 653819726150: Mather JP, Phillips DM. Establishment of a peritubular myoid-like cell line and interactions between established testicular cell lines in culture. J. Ultrastruct. Res. 87: 263-274, 1984. PubMed: 6544874 |
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