BRL 3A大鼠肝细胞

产品介绍

目录号 Serial	GNR10
标识符 Identifier	CSTR:19375.09.3101RATGNR10
描述 Description	这个细胞株的无血清条件培养基是MSA的一个来源。 MSA多肽家族能够部分满足鸡胚成纤维细胞对血清的需求。在本库通过支原体检测。
动物种别 Organism	大鼠
性别 Gender	***
组织来源 Tissue and Cell Type	肝
形态 Morphology	***
培养基和添加剂 Complete Growth Medium and Culture Conditions	DMEM培养基(GIBCO by life technologies ,货号C11995500BT)，90%；胎牛血清，10%。气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	Nissley SP , et al. Proliferation of buffalo rat liver cells in serum-free medium does not depend upon multiplication-stimulating activity (MSA). Cell 11: 441-446, 1977. PubMed: 302146Coon HG , Weiss MC . A quantitative comparison of formation of spontaneous and virus- produced viable hybrids. Proc. Natl. Acad. Sci. USA 62: 852-859, 1969. PubMed: 4308097Dulak NC , Temin HM . A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts. J. Cell. Physiol. 81: 153-170, 1973. PubMed: 4735141Dulak NC , Shing YW . Large scale purification and further characterization of a rat liver cell conditioned medium multiplication stimulating activity. J. Cell. Physiol. 90: 127-130, 1977. PubMed: 833209Schalch DS , et al. Nonsuppressible insulin-like activity (NSILA). I. Development of a new sensitive competitive protein-binding assay for determination of serum levels. J. Clin. Endocrinol. Metab. 46: 664-671, 1978. PubMed: 755052

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