NIH/3T3小鼠胚胎成纤维细胞

产品介绍

目录号 Serial	GNM 6
标识符 Identifier	CSTR:19375.09.3101MOUGNM6
描述 Description	与原始的随机交配3T3和近交系BALB/c 3T3建株方法一样，NIH/3T3，是从NIH Swiss小鼠胚胎培养物中建立的高度接触抑制的连续细胞株。为了培育在形态学特征上更适合于进行转化分析的亚株，建立的NIH/3T3细胞株又进行了五轮以上亚克隆。这株细胞对DNA转化及转染研究十分有用。检测表明肢骨发育畸形病毒(鼠痘)阴性。在本库通过支原体检测。
动物种别 Organism	小鼠
性别 Gender	***
组织来源 Tissue and Cell Type	胚胎
形态 Morphology	成纤维细胞
培养基和添加剂 Complete Growth Medium and Culture Conditions	DMEM培养基(GIBCO by life technologies ,货号C11995500BT)，90%；优质胎牛血清，10%。气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
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