NIH/3T3小鼠胚胎成纤维细胞(小鼠,品系NIH/Swiss)

产品介绍

目录号 Serial	SCSP-515
标识符 Identifier	CSTR:19375.09.3101MOUSCSP515
描述 Description	NIH/3T3是从小鼠NIH/Swiss品系的胚胎中建立的成纤维细胞株。该细胞易于转染，对肉瘤病毒转化灶形成和白血病病毒增殖十分敏感。据报道，鼠痘病毒(ectromelia virus，ECTV)检测结果为阴性。细胞质控结果： 1.支原体检测结果：每个批次均通过本库支原体检测，结果为阴性。 2.STR鉴定结果： ①该株细胞DNA进行小鼠细胞STR分型结果显示，扩增后图谱清晰，分型结果良好: 1-1: 10；1-2: 13, 17；2-1: 9；3-2: 14, 15；4-2: 19.3, 20.3；5-5: 14, 15；6-4: 15.3；6-7: 12；7-1: 29；8-1: 15；11-2: 15, 17；12-1: 20；13-1: 16.2；15-3: 20.3；17-2: 13, 14；18-3: 17, 19；19-2: 11, 12；X-1: 25。 ②该细胞与ExPASy中NIH 3T3细胞的STR数据匹配率为100%。 ③该株细胞确为小鼠细胞，没有人源细胞污染。
动物种别 Organism	小鼠，品系NIH/Swiss
性别 Gender
组织来源 Tissue and Cell Type	胚胎
形态 Morphology	成纤维细胞，贴壁生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	NIH/3T3 完全培养液配方(100 ml) DMEM (Invitrogen, 11960-044)88 ml 新生牛血清 (Gibco)灭活后使用 10 ml Glutamax (Invitrogen, 35050061) 1 ml Sodium Pyruvate 100 mM Solution (Invitrogen, 11360070) 1 ml 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。【注意事项：】 NIH/3T3细胞对于血清较为敏感，不能使用胎牛血清(FBS)培养该细胞。生物安全等级：BSL-1 参考传代周期：2～3天参考传代比例：1：4(备注：切勿使细胞生长过密，每周至少传代2次，保证细胞密度不超过80%。) 参考换液频率：2～3天冻存液配方：完全培养液95%，DMSO 5%
供应限制 Permits and Restrictions	A。仅供研究之用。
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