TCCSUP人膀胱癌细胞(2014年新引进)

产品介绍

目录号 Serial	SCSP-571
标识符 Identifier	CSTR:19375.09.3101HUMSCSP571
描述 Description	人膀胱癌细胞。该患者在切除肿瘤之前有4个月的血尿病史。后来发现了向骨髓的转移。超微结构研究表明存在微绒毛和脂质体，但未观察到桥粒。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测无误。STR鉴定结果为：Amelogenin: X; CSF1PO: 10; D13S317: 11,14; D16S539: 9,11; D5S818: 12; D7S820: 8,9; THO1: 6,9.3; TPOX: 8; vWA: 14,16
动物种别 Organism	人
性别 Gender	女性，67岁
组织来源 Tissue and Cell Type	膀胱
形态 Morphology	上皮样，贴壁生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	人膀胱癌细胞 TCCSUP 完全培养液配方(100 ml) EMEM (Invitrogen，11090081) 87 ml FBS (Gibco)10 ml Glutamax(invitrogen 35050061) 1 ml Non-essential Amino Acids, 100x (Invitrogen, 11140050) 1 ml Sodium Pyruvate 100 mM Solution(invitrogen 11360070) 1 ml 参考传代周期：6-8天1次参考传代比例：1:2-1:3 参考换液频率：每周2-3次冻存液配方：完全培养液95%，DMSO 5% 细胞状态：贴壁生长气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484Nayak SK, et al. A cell line from an anaplastic transitional cell carcinoma of human urinary bladder. Br. J. Cancer 35: 142-151, 1977. PubMed: 836756

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